Glycoenzymes for Bioindustries
About Glycoenzymes for Bioindustries
Glycoenzymes are responsible for the biosynthesis of all glycans and glycoconjugates and as such provide a rich source of biocatalysts for industrial applications; however, few glycoenzymes are commercially available.
This project will translate existing academic work in the production of an expanded toolkit of glycoenzymes suitable for transformational potential in: diagnostics, biologics and nutraceuticals, but with broader applications which can be satisfied via enzyme production and supply partners.
The project requires an integrated approach featuring:
Identification of desirable glycoenzyme activities
Identification of candidate genes/enzymes using advanced data-mining techniques
Standardising, benchmarking and generating an invaluable open access database
Optimisation of biocatalysts in terms of stability, activity, production scale and substrate specificity
Establishing novel high-throughput screening protocols that are benchmarked against existing methods
Enabling commercial production of glycoenzymes at gram and kg scale
Realisation of this project will lead to the generation of glycoenzymes which can be directly commercialised as a toolkit for glycoscience and Industrial Biotechnology applications, and evaluated for novel advanced manufacturing applications.
Project Lead: The University of Manchester│Professor Sabine Flitsch & Professor Nicholas Turner
Project Partner: John Innes Centre│Professor Rob Field
Project Partner: Newcastle University│Dr David Bolam
Project Partner: Institute of Food Research│Dr Nathalie Juge
Commerical Partner: Ludger Ltd
Commerical Partner: Biocatalysis Ltd
Subcontractor: Prozomix Ltd